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目的 探讨SH3域结合谷氨酸丰富蛋白样蛋白(SH3BGRL)的表达水平对血管紧张素Ⅱ(AngⅡ)诱导的心肌细胞肥大的影响。方法 通过构建H9C2心肌细胞肥大模型，运用Western blot和RT-PCR技术分别检测SH3BGRL水平，RT-PCR技术检测心肌肥大相关标志基因ANP、BNP和β-MHC的表达情况。结果 过表达SH3BGRL后，心肌肥大相关标志基因的mRNA表达水平升高(P<0.05);而沉默SH3BGRL后，mRNA表达水平显著降低(P<0.05)。结论 SH3BGRL的表达水平与心肌肥大标志基因的表达呈正相关，有望成为治疗心肌肥大的潜在靶点，为深入研究SH3BGRL在心脏中的功能奠定了坚实基础。
Abstract：Objective To investigate the effect of SH3 domain-binding glutamate-rich protein-like protein(SH3BGRL) expression on cardiomyocyte hypertrophy induced by angiotensin Ⅱ(Ang Ⅱ).Methods A hypertrophy model of H9C2 cardiomyocytes was constructed.Western blot and RT-PCR were used to detect the expression level of SH3BGRL,and RT-PCR was used to detect the expression of cardiac hypertrophy-related marker genes ANP,BNP,and β-MHC.Results The mRNA expression levels of cardiac hypertrophy-related marker genes significantly increased after overexpression of SH3BGRL(P<0.05).Conversely, the mRNA expression levels of cardiac hypertrophy-related marker genes significantly decreased after silencing SH3BGRL(P<0.05).Conclusion The expression level of SH3BGRL is positively correlated with the expression of cardiac hypertrophy marker genes, suggesting that it could be a potential target for treating myocardial hypertrophy and laying a solid foundation for further research into the function of SH3BGRL in the heart.
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基本信息:
DOI：10.16751/j.cnki.2095-4646.2025042916
中图分类号:R54
引用信息:
[1]高润泽,魏小茗,韩婉婷,等.SH3BGRL的表达对AngⅡ诱导的H9C2心肌细胞肥大的影响[J].湖北科技学院学报(医学版),2026,40(01):44-48.DOI:10.16751/j.cnki.2095-4646.2025042916.
基金信息:
湖北省自然科学基金(2024AFB1025); 湖北科技学院博士启动基金(BK202414);湖北科技学院医学科研专项基金(2022YKY18)
2026-01-20
2026-01-20