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目的 以荷叶原粉为原料，采用酵母菌发酵法提取荷叶多糖，探究最佳发酵条件及所提取荷叶多糖的抗氧化活性。方法 以荷叶多糖得率为响应值，采用单因素实验和响应面优化确定最佳发酵条件；采用紫外分光光度法测定荷叶多糖对2,2-联苯基-1-苦基肼基(DPPH·)和羟自由基(·OH)的清除率。结果 酵母菌发酵提取荷叶多糖的最佳工艺条件为：发酵温度27.6℃,发酵时间48h,酵母添加量2.6%,液料比26 mL/g。该条件下粗多糖的提取率为4.19%,多糖含量为19.30%;荷叶多糖对DPPH·和·OH的清除率分别为73.3%、93.4%。结论 酵母菌发酵法不仅能够提高荷叶多糖的提取率，而且能够增强其抗氧化活性，为荷叶多糖的工业化提取提供参考。
Abstract：Objective To optimize the fermentation conditions for extracting polysaccharides from lotus leaf powder and to evaluate the antioxidant activity of the obtained polysaccharides.Methods Using the yield of lotus leaf polysaccharide as the response value, the optimum fermentation conditions were determined by single factor experiments and response surface methodology.The scavenging rate of lotus leaf polysaccharide against 2,2-diphenyl-1-picrylhydrazyl(DPPH·) and hydroxyl radical(·OH) were determined by ultraviolet spectrophotometry.Results The optimal conditions for the extraction of lotus leaf polysaccharide by yeast fermentation were as follows: fermentation temperature 27.6 ℃,fermentation time 48 h, yeast addition 2.6 %,and liquid-solid ratio 26mL/g.Under these conditions, the extraction rate of crude polysaccharide was 4.19 %,with a polysaccharide content of 19.30%.The scavenging rates of DPPH·and ·OH were 73.3% and 93.4 %,respectively.Conclusion The yeast fermentation method can not only improve the extraction rate of lotus leaf polysaccharide, but also enhance its antioxidant activity, providing a reference for the industrial extraction of lotus leaf polysaccharide.
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基本信息:
DOI：10.16751/j.cnki.2095-4646.2025041710
中图分类号:R284.2
引用信息:
[1]曲晓营,邢宇畅,柴爽,等.荷叶多糖的酵母菌发酵提取及体外抗氧化活性研究[J].湖北科技学院学报(医学版),2026,40(01):12-16+28.DOI:10.16751/j.cnki.2095-4646.2025041710.
基金信息:
国家自然科学基金(21972108); 湖北省中央引导地方科技发展专项(2024CSA085); 湖北省教育厅科学技术研究项目(B2022184,BXLBX0780,S202110927039)
2025-04-17
2025
2026-01-16
2026
2
2025-11-28
2025-11-28
2025-11-28