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目的 研究刺梨不同药用部位乙醇提取物的清除自由基活性。方法 通过对刺梨不同部位乙醇提取物中总酚、总黄酮的含量进行测定，同时对其清除DPPH、ABTS以及·O~-2的活性进行分析。结果 刺梨(果实)中，去籽刺梨(DR)总酚含量(234.17±4.00)μg/mg显著高于其他部位(P<0.05)。而整果残渣(WRR)、去籽刺梨残渣(DRR)总黄酮含量分别为(21.05±0.78)μg/mg、(24.42±3.71)μg/mg,均显著高于果实其他部位。活性方面，刺梨整果(WR)与DR对DPPH、ABTS自由基清除活性显著优于果实其他部位(P<0.05),各个部位乙醇提取物DPPH、ABTS自由基清除活性与其总酚含量呈剂量依赖关系。而·O~-2清除实验中WR与DR清除活性优于阳性药2,6-二叔丁基-4-甲基苯酚(BHT)(P<0.05)。结论 去籽刺梨(果肉)是果实包含总酚与发挥清除自由基活性的主要部位，可优先作为进一步研究刺梨抗氧化、抗炎等活性的药用部位。
Abstract：Objective To explore the free radical scavenging activity of ethanol extracts from different medicinal parts of Rosa roxburghii.Methods The total phenolic and total flavonoid contents in ethanol extracts from different parts of R.roxburghii were determined, and the scavenging activities against DPPH,ABTS,and·O~-_2were analyzed.Results In the fruit of R.roxburghii,the total phenolic content of seedless R.roxburghii(DR)(234.17±4.00)μg/mg was significantly higher than that of other parts(P<0.05).The total flavonoid contents of whole fruit residue(WRR) and seedless R.roxburghii residue(DRR) were(21.05±0.78)μg/mg and(24.42±3.71)μg/mg, respectively, which were significantly higher than those of other parts in the fruit.In terms of activity, the whole fruit(WR) and DR had significantly better scavenging activities against DPPH and ABTS free radicals than other parts of the fruit(P<0.05).The scavenging activities of DPPH and ABTS free radicals of ethanol extracts from each part were in a dose-dependent relationship with their total phenolic contents.In the ·O~-2 scavenging experiment, the scavenging activity of WR and DR was better than that of the positive drug butylated hydroxytoluene(BHT)(P<0.05).Conclusion Seedless R.roxburghii(fruit flesh) is the main part of the fruit that contains total phenolic and has the ability to scavenge free radicals.It can be prioritized as a medicinal part for further research on the antioxidant and anti-inflammatory activities of R.roxburghii.
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基本信息:
DOI：10.16751/j.cnki.2095-4646.2025052310
中图分类号:R284.2
引用信息:
[1]程文敬,万哲,管志威,等.刺梨不同药用部位乙醇提取物清除自由基的活性研究[J].湖北科技学院学报(医学版),2026,40(01):23-28.DOI:10.16751/j.cnki.2095-4646.2025052310.
基金信息:
湖北科技学院糖尿病心脑血管病变重点实验室糖尿病专项基金(2022TNB06);湖北科技学院校内博士科研启动项目(BK202332)
2026-01-20
2026-01-20