湖北科技学院学报（医学版）

目的观察大蒜多糖A、B、C对四氯化碳(CCl4)肝损伤小鼠血清和肝组织丙氨酸转氨酶(alanine aminotransferase,ALT)、天冬氨酸转氨酶(aspartate aminotransferase,AST)的影响及其急性毒性测定。方法从大蒜球根中提取有效成分大蒜多糖A、B、C,并测定口服给药LD50及急性毒性限量以观察三者急性毒性;采用CCl4灌胃建立小鼠实验性肝损伤模型,测定小鼠血清和肝组织ALT、AST。结果小鼠口服大蒜多糖A、B、C,均未测出LD50,大蒜多糖A、B、C急性毒性限量试验,安全无毒。大蒜多糖A、B、C治疗使CCl4肝损伤小鼠血清ALT及AST活性显著降低(P<0.01),肝组织ALT及AST活性显著增高(P<0.01);其中,以大蒜多糖C作用最强,优于大蒜素;B次之,与大蒜素相似;A作用最弱,弱于大蒜素。结论大蒜多糖A、B、C均有护肝作用,以大蒜多糖C作用最佳,安全无毒。

Objective To study the effects of the allium sativum L polysaccharide A,B and C on the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in injured mice serum or Liver and to detect the Acute Toxicity. Methods The Allium sativum L polysaccharide A,B and C were extract from garlic root-stem. The acute toxicity were observed by medial lethal dose(LD50)and acute maximal tolerance dose toxicity testing. The experimental liver injury model was reestablished by 500 mL/L carbon tetrachloride solution(0. 1ml/10g,ig). ALT and AST in serum or liver were assayed by biochemical method. Results LD50 of the Allium sativum L polysaccharide A,B and C on mice for oral administration could not be detected. The acute maximal tolerance dose toxicity testing found that the Allium sativum L polysaccharide A,B and C (5g/kg·d ig×14) didn't show obvious toxicity on mice. Allium sativum L polysaccharide A,B and C(0.6g/kg. d ig×10) could obviously reduce the activities of ALT and AST in serum and increase activities of ALT and AST in liver in carbon tetrachloride (CCL4)-treated mice(P<0. 01), and the action of three components were gradually increased in order. For the effects on the ALT and AST, component C were most powerful and stronger than allitridi, component B were less powerful and similar with allitridi, while component A were inferior to allitridi. Conclusion The allium sativum L polysaccharide A, B and C were all the new effective liver protection ingredient in garlic with less toxicity,the allium sativum L polysaccharide C show more powerful liver protecting activity.