| 361 | 0 | 137 |
| 下载次数 | 被引频次 | 阅读次数 |
目的 探讨青砖茶提取物(QDTE)对葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎(UC)小鼠的保护作用及其潜在机制。方法 采用3%DSS诱导C57BL/6小鼠建立UC模型，并分别给予200mg/(kg·d)和400mg/(kg·d)的QDTE进行干预。通过监测体重变化、疾病活动指数(DAI)及结肠长度评估QDTE的治疗效果；采用酶联免疫吸附试验(ELISA)检测血清和结肠组织中炎症因子(IL-6、TNF-α)水平；利用免疫组化(IHC)分析紧密连接蛋白-1(ZO-1)、闭合蛋白(Occludin)的表达变化。结果 与Model组相比，QDTE干预显著缓解了UC小鼠体重下降(P<0.05),降低了DAI评分(P<0.05),增加结肠长度(P<0.05)。此外，QDTE显著抑制了结肠及血清中促炎因子IL-6和TNF-α的释放(P<0.05),同时上调了肠黏膜ZO-1和Occludin的表达(P<0.05)。结论 QDTE通过抑制炎症反应、增强肠道屏障功能，有效缓解DSS诱导的小鼠UC症状，表明其具有作为功能性食品或辅助治疗UC的潜在应用价值。
Abstract：Objective To investigate the therapeutic effects and potential mechanism of Qingzhuan Dark tea extract(QDTE) on ulcerative colitis(UC) in mice induced by dextran sulfate sodium(DSS).Methods A UC model was established in C57BL/6 mice by 3% DSS,and the mice were intervened with 200mg/(kg·d) and 400mg/(kg·d) of QDTE,respectively.The therapeutic effect of QDTE was evaluated by monitoring body weight changes, disease activity index(DAI),and colon length.The levels of inflammatory factors(IL-6,TNF-α) in serum and colon tissue were detected by enzyme-linked immunosorbent assay(ELISA),and the expression changes of zonula occludens-1(ZO-1) and occludin were analyzed by immunohistochemistry.Results Compared with the Model group, QDTE intervention significantly alleviated the weight loss of UC mice(P<0.05),reduced DAI scores(P<0.05),and increased colon length(P<0.05).In addition, QDTE significantly inhibited the release of pro-inflammatory factors IL-6 and TNF-α in the colon and serum(P<0.05),and upregulated the expression of ZO-1 and Occludin in the intestinal mucosa(P<0.05).Conclusion QDTE effectively alleviates the symptoms of DSS-induced UC in mice by inhibiting inflammatory responses and enhancing intestinal barrier function, indicating its potential application value as a functional food or adjuvant therapy for UC.
[1]ZHOU Y,XU H,XU J,et al.Prausnitzii and its supernatant increase SCFAs-producing bacteria to restore gut dysbiosis in TNBS-induced colitis[J].AMB Express,2021,11(1):33
[2]PEDERSEN K M,?OLAK Y,VEDEL-KROGH S,et al.Risk of ulcerative colitis and Crohn's disease in smokers lacks causal evidence[J].Eur J Epidemiol,2022,37(7):735
[3]LI M,YANG L,MU C,et al.Gut microbi-al metabolome in inflammatory bowel disease:from association to therapeutic perspectives[J].Comput Struct Biotechnol J,2022,20:2402
[4]TAN Y,GUAN Y,SUN Y,et al.Correlation of intestinal mucosal healing and tight junction protein expression in ulcerative colitis patients[J].Am J Med Sci,2019,357(3):195
[5]TOSCHI A,PIVA A,GRILLI E,et al.Botanicals modulate oxidative stress and epithelial integrity in intestinal epithelial cells[J].Animals (Basel),2022,12(17):2188
[6]HUANG Y,XING K,QIU L,et al.Therapeutic implications of functional tea ingredients for ameliorating inflammatory bowel disease:a focused review[J].Crit Rev Food Sci Nutr,2021,62(19):5307
[7]ZHENG K,ZHAO Q,CHEN Q,et al.The synergic inhibitory effects of dark tea (camellia sinensis) extract and p38 inhibition on the growth of pancreatic cancer cells[L].J Cancer,2019,10(26):6557
[8]ZHU J,WU M,ZHOU H,et al.Liubao brick tea activates the PI3K-Akt signaling pathway to lower blood glucose,metabolic disorders and insulin resistance via altering the intestinal flora[J].Food Res Int Ott Ont,2021,148:110594
[9]]LIU H,CHEN R,WEN S,et al.Tea (Camellia sinensis) ameliorates DSS-induced colitis and liver injury by inhibiting TLR4/NF-κB/NLRP3 inflammasome in mice[J].Biomed Pharmacother,2023,158:114136
[10]ZHANG Y,FENG X,LIN H,et al.Tieguanyin extracts ameliorated DSS-induced mouse colitis by suppressing inflammation and regulating intestinal microbiota[J].Food Funct,2022,13(24):13040
[11]AGARWAL R,KATIYAR S K,ZAIDI S I,et al.Inhibition of skin tumor promoter-caused induction of epidermal ornithine decarboxylase in SENCAR mice by polyphenolic fraction isolated from green tea and its individual epicatechin derivatives[J].Cancer Res,1992,52(13):3582
[12]ZHAO Y,CHEN H,LI W,et al.Selenium-containing tea polysaccharides ameliorate DSS-induced ulcerative colitis via enhancing the intestinal barrier and regulating the gut microbiota[J].Int J Biol Macromol,2022,209(Pt A):356
[13]ZHANG L,XIONG S,JIN F,et al.Global trends in intestinal flora and ulcerative colitis research during the past 10 years:a bibliometric analysis[J].Front Microbiol,2022,13:1003905
[14]谭悦，郑长清.紧密连接蛋白occludin、ZO-1在溃疡性结肠炎中的表达及其临床意义[J].现代药物与临床，2018,33(7):1803
[15]TONG L C,WANG Y,WANG Z B,et al.Propionate ameliorates dextran sodium sulfate-induced colitis by improving intestinal barrier function and reducing inflammation and oxidative stress[J].Front Pharmacol,2016,7:253
[16]KANG S,NARAZAKI M,METWALLY H,et al.Historical overview of the interleukin-6 family cytokine[J].J Exp Med,2020,217:e20190347
基本信息:
DOI：10.16751/j.cnki.2095-4646.2025030707
中图分类号:TS272.54;R574.62
引用信息:
[1]赵依叶,叶丹,权浩洋,等.青砖茶提取物对溃疡性结肠炎小鼠治疗作用研究[J].湖北科技学院学报(医学版),2025,39(06):466-470.DOI:10.16751/j.cnki.2095-4646.2025030707.
基金信息:
国家自然科学基金重大科研仪器研制项目(81727805); 咸宁市科技研发重点专项项目(2021SFYF003); 湖北科技学院科研创新团队项目(2023T10)