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目的 探讨山奈酚(Kae)改善自发性高血压大鼠(SHR)肾纤维化的作用机制。方法 将大鼠分为4组，分别为CON组、SHR组、SHR+Los(氯沙坦)组和SHR+Kae(山奈酚)组，每组8只。然后分别进行灌胃处理，早晚各一次，连续灌胃4 w后测定血压及取肾组织；将大鼠肾组织分别进行H&E、PAS和DHE染色，Western blot检测纤维化相关蛋白及NRF2蛋白的表达。结果 与Con组相比，SHR组大鼠肾组织呈明显纤维化改变，ROS生成增多，胶原相关蛋白表达明显升高(P<0.001),NRF2表达明显降低(P<0.01)。给予Kae处理后，大鼠血压降低，肾组织纤维化得到明显改善，ROS生成明显减少，NRF2表达升高。结论 山奈酚可通过缓解氧化应激改善自发性高血压大鼠的肾纤维化。
Abstract：Objective To explore the mechanism by which kaempferol(Kae) ameliorates renal fibrosis in spontaneously hypertensive rats(SHR).Methods Rats were divided into four groups(n=8 per group):CON group, SHR group, SHR+Los(Losartan) group, and SHR+Kae group.The rats were administered the corresponding treatments by gavage twice daily(morning and evening) for four consecutive weeks.After that, blood pressure was measured and renal tissues were collected.Renal tissues were stained with H&E,PAS and DHE,and Western blot was used for detecting the expression of fibrosis-related proteins and NRF2 protein.Results Compared with the CON group, the renal tissues of the SHR group showed obvious fibrotic changes, increased ROS production, and significantly elevated expression of collagen-related proteins(P<0.001),and significantly decreased expression of NRF2(P<0.01).After Kae treatment, the blood pressure of rats decreased, renal fibrosis was significantly improved, ROS production was significantly reduced, and the expression of NRF2 increased.Conclusion Kaempferol can improve renal fibrosis in spontaneously hypertensive rats by alleviating oxidative stress.
[1]YAO M,LIAN D,WU M,et al.Isoliensinine attenuates renal fibrosis and inhibits TGF-β1/Smad2/ 3Signaling pathway in spontaneously hypertensive rats[J].Drug Des Devel Ther,2023,17:2749
[2]LIU Y.Cellular and molecular mechanisms of renal fibrosis[J].Nat Rev Nephrol,2011,7(12):684
[3]TIENDA-VAZQUEZ M A,MORREEUW Z P,SOSA-HERNáNDEZ J E,et al.Nephroprotective plants:a review on the use in pre-renal and post-renal diseases[J].Plants (Basel),2022,11(6):818
[4]LAN X,WANG Q,LIU Y,et al.Isoliquiritigenin alleviates cerebral ischemia-reperfusion injury by reducing oxidative stress and ameliorating mitochondrial dysfunction via activating the Nrf2 pathway[J].Redox Biol,2024,77:103406
[5]ALSAWAF S,ALNUAIMI F,AFZAL S,et al.Plant flavonoids on oxidative stress-mediated kidney inflammation[J].Biology (Basel),2022,11(12):1717
[6]WU H,DU J,LI C,et al.Kaempferol can reverse the 5-fu resistance of colorectal cancer cells by inhibiting PKM2-mediated glycolysis[J].Int J Mol Sci,2022,23(7):3544
[7]WANG J,FANG X,GE L,et al.Antitumor,antioxidant and anti-inflammatory activities of kaempferol and its corresponding glycosides and the enzymatic preparation of kaempferol[J].PLoS One,2018,13(5):e0197563
[8]YUE Z,ZHANG Y,ZHANG W,et al.Kaempferol alleviates myocardial ischemia injury by reducing oxidative stress via the HDAC3-mediated Nrf2 signaling pathway[J].J Adv Res,2025,75:755
[9]WANG Z,SUN W,SUN X,et al.Kaempferol ameliorates cisplatin induced nephrotoxicity by modulating oxidative stress,inflammation and apoptosis via ERK and NF-κB pathways[J].AMB Express,2020,10(1):58
[10]JI X,CAO J,ZhANG L,et al.Kaempferol protects renal fibrosis through activating the BMP-7-Smad1/5 signaling pathway[J].Biol Pharm Bull,2020,43(3):533
[11]DABEEK W M,MARRA M V.Dietary quercetin and kaempferol:bioavailability and potential cardiovascular-related bioactivity in humans[J].Nutrients,2019,11(10):2288
[12]ALKANDAHRI M Y,SADINO A,PAMUNGKAS B T,et al.Potential nephroprotective effect of kaempferol:biosynthesis,mechanisms of action,and clinical prospects[J].Adv Pharmacol Pharm Sci,2024,2024:8907717
[13]SHARMA N,BISWAS S,Al-DAYAN N,et al.Antioxidant role of kaempferol in prevention of hepatocellular carcinoma[J].Antioxidants (Basel),2021,10(9):1419
[14]ALSHEHRI A S,EL-KOTT A F,EL-KENAWY A E,et al.The ameliorative effect of kaempferol against CdCl2- mediated renal damage entails activation of Nrf2 and inhibition of NF-kB[J].Environ Sci Pollut Res Int,2022,29(38):57591
基本信息:
DOI：10.16751/j.cnki.2095-4646.2025040814
中图分类号:R965
引用信息:
[1]蒋越,李奥迪,徐甲淇,等.山奈酚缓解氧化应激改善自发性高血压大鼠肾纤维化[J].湖北科技学院学报(医学版),2025,39(06):476-479.DOI:10.16751/j.cnki.2095-4646.2025040814.
基金信息:
湖北省自然科学基金咸宁创新发展联合基金(2025AFD88)