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目的 探讨白桦脂酸(BA)抑制宫颈癌SIHA细胞的恶性生物学行为及部分分子机制。方法 在恒温恒湿CO2细胞培养箱中培养宫颈癌SIHA细胞，将细胞与不同浓度梯度的BA孵育后进行细胞增殖和迁移检测。采用MTT法检测SIHA细胞的光密度值，接着评价同一浓度下BA对SIHA细胞抑制的时间依赖性；平板克隆形成实验检测宫颈癌SIHA细胞的单细胞克隆增殖能力；通过Transwell小室迁移和侵袭实验来评估宫颈癌SIHA细胞的迁移能力；蛋白质免疫印迹(Western blot)实验检测BA影响宫颈癌SIHA细胞中c-Myc蛋白表达水平的情况；Rescue实验检测BA是否通过c-Myc通路介导其抑制作用。结果 BA可以剂量和时间依赖性地抑制宫颈癌SIHA细胞的存活和增殖能力，浓度依赖性地抑制SIHA细胞的迁移和侵袭能力；在一定剂量范围内，BA可以引起癌基因c-Myc蛋白质表达下调，c-Myc-saRNA可以部分抵消BA的抑制作用。结论 BA对宫颈癌SIHA细胞的增殖与迁移具有抑制作用，其作用机制与下调c-Myc蛋白表达有关。
Abstract：Objective To evaluate the inhibitory effect and partial molecular mechanism of betulinic acid(BA) on the malignant biological behavior of cervical cancer SIHA cells.Methods Cervical cancer SIHA cells were cultured in cell incubator with constant temperature and humidit and treated with different concentration gradients of BA to detecte the proliferation and migration ability.The optical-density of SIHA cells was detected by MTT assay, and then the time-dependent inhibition of SIHA cells by BA at the same concentration was evaluated.The single cell clonal proliferation ability of SIHA cells was detected by plate clone formation assay.The migration and invasion ability of SIHA cells were evaluated by Transwell cell small chamber migration and invasion assay.The c-Myc protein expression level in SIHA cells treated with BA was deteced by Western blot assay.Resue assay was applied to demonstrate whether the inhibitory effect of BA was mediated by c-Myc pathway.Results BA could inhibit the survival and proliferation of SIHA cells in a time and dose-dependent manner, as well as the migration and invasion in a dose-dependent manner.In a certain dose range, BA down-regulated the expression of oncogene c-Myc protein.c-Myc-saRNA can partly counteract the inhibition effect of BA.Conclusion BA can inhibit the proliferation and migration of cervical cancer SIHA cells, and its mechanism is related to the down-regulation of c-Myc protein expression.
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基本信息:
DOI：10.16751/j.cnki.2095-4646.2025010610
中图分类号:R965
引用信息:
[1]祝巧云,朱波.白桦脂酸通过下调c-Myc抑制宫颈癌SIHA细胞的增殖与迁移[J].湖北科技学院学报(医学版),2025,39(05):386-390.DOI:10.16751/j.cnki.2095-4646.2025010610.